3, 3',5 ,5'-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA, ready to use solution

Synonyms: TMB

MDL No.: MFCD00007748

Storage: 2-8C

UNSPSC Code: 12352204

Application: Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Application: 3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.