Synonym(s): Factor IIa
CAS No.: 9002-04-4
Analysis Note: The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Storage: −20°C
EC No.: 232-648-7
Biochem/physiol Actions: Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Reconstitution: When reconstituted with 1 mL water, vial contains stated activity in 0.15 M sodium chloride and 0.05 M sodium citrate, pH 6.5.
Unit Definition: Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
Empirical Formula (Hill Notation): C12H8N2