Synonyms: TRIS HCl; TRIS hydrochloride; Tris(hydroxymethyl)aminomethane hydrochloride; Tromethane hydrochloride
Molecular Formula: C4H11NO3 · HCl
Molecular Weight: 157.6
Linear Structural Formula: NH2C(CH2OH)3 · HCl
MDL Number: MFCD00012590
Purity: >=99.0% (titration)
Melting Point: 150-152 C
Application: The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information: Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes: Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Abs.: A40%/290 ≤0.05
Assay: ≥99.0% (titration)
Cation Traces: heavy metals (as Pb): ≤5 ppm
Grade: for molecular biology
Impurities: ≤0.5% water (Karl Fischer)
MP: 150-152°C
pKa (25°C): 8.1
Quality Level: ELITE
Solubility: H2O: soluble667 mg/mL
Suitability: suitable for electrophoresis
Useful pH Range: 7.0 - 9.0