Cleavage of the oligonucleotide from its solid support and subsequent removal of all protecting groups from the nucleobases and phosphates close the cycle of automated oligonucleotide synthesis and bring it to completion. For this purpose, three different Cleavage solutions from emp BIOTECH are available. The correct choice will depend on your requirements for standard, fast or mild cleavage conditions.
Standard: Usage of one volume of conc. ammonium hydroxide (≥ 28 %) under sealed conditions appropriate for removal of the protecting groups of the nucleobases Fast: Usage of one volume of AMA under sealed conditions at 65 °C for 10 min Mild: Usage of the required volume of Deprotection, Ultramild under sealed conditions for 8 h at room temperature