Synonyms: Fibronectin
MDL No.: MFCD00131062
Storage: 2-8C
UNSPSC Code: 12352200
General description: Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. The FN1 gene mapped to human chromosome 2q35.
Application: Fibronectin human plasma has been used in the determination of H2O2 consumption rate. It has also been used in coating the Sylgard(R) models for cell culture. It has also been used in cell adhesion and to directly pattern the protein onto the polymeric film.
Biochem/physiol Actions: Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis.
Analysis Note: Protein homogeneity is evaluated by immunoelectrophoresis.
Preparation Note: This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C. In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8C
RIDADR: NONH for all modes of transport
WGK Germany: 1