iSWAB-Microbiome (MB) is a non-toxic stabilizing technology that enables ambient collection and transport of various biosamples, and maintains the status quo at the time of collection. iSWAB-MB has been proven to efficiently inactivate bacteria, fungi, spores, and DNA/RNA viruses allowing safer transport of biological samples to processing labs, especially when samples are subject to long transit times. The buffer does not contain guanidine or related compounds. DNA and RNA from collected microorganisms remains stable at room temperature for up to 40 days for DNA, and 21 days for RNA, eliminating the need for expedited cold chain transport. SWAB-MB provides a representative snapshot of the microbial community that remains unchanged from collection to processing of oral, gut/fecal, skin, vaginal, and soil samples. Purified microbial DNA or RNA isolated from collected samples are compatible with qPCR, microarray, and NGS for microbiome research in health, wellness, and forensics.