Antibodix columns are specially designed for high resolution, high efficiency and high recovery separations of antibodies. The packing support is composed of a rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) non-porous bead. The non-porous resin has particle size of 1.7, 3, 5 and 10 µm. The PS/DVB resin surface is grafted with a highly hydrophilic, neutral polymer thin layer with the thickness in the range of nanometer. On the top of the hydrophilic layer, weak cation-exchange functional groups are attached via a proprietary chemistry, resulting in high capacity ion-exchange layer.