CHU (N6) Medium has been developed by C.C. et al. in 1975 for the in vitro anther culture of oryza sativa family Graminae. The medium helps in the initiation, growth and differentiation of callus from the rice pollen cultures. CHU (N6) Medium is a nutrient blend of inorganic salts that consists of macroelements, microelements, vitamins and amino acid. Potassium nitrate serves as a source of nitrate. Ammonium sulphate quantity has been reduced as ammonium ions have inhibitory effect on growth and quality of the rice callus but aids in morphogenesis. Microelements like Boron, Manganese, Molybdenum, Copper, Iron and Zinc play vital role in plant metabolism and improves the quality of anther callus. Thiamine, pyridoxine, nicotinic acid act as enzymatic cofactors in universal pathways including glycolysis and TCA cycle along with the primary and secondary metabolism in the plants. The product is plant tissue culture tested but it is the sole responsibility of the user to ensure the suitability of the medium for individual species.
Application: For the in Vitro Anther Culture of oryza Sativa Family Graminae. The Medium Helps in the initation,Growth and Differentiation of Callus from the Rice Pollen Cultures
- Appearance : White to off-white, homogeneous, free flowing powder
- Solubility: 4.0 gms/litre soluble in distilled water
- Color and Clarity: Colorless to light yellow, clear solution