Gamborg B5 medium has been established by Gamborg O.L (1968) for the callus and cell suspension culture of Glycine max, family Fabaceae. This medium is widely used for in vitro plant cell, tissue and organ culture. Gamborg B5 medium is a nutrient blend of inorganic salts, vitamins and carbohydrate. Potassium nitrate serves as a sole source of nitrate as increased nitrate content is beneficial for soyabean root callus and ammonium sulphate enhances the cell growth. Sodium dihydrogen phosphate serves as a phosphate source and the microelements like Boron, Manganese, Molybdenum, Copper, Iron and Zinc play vital role in plant metabolism. Boron plays a key role in carbohydrate metabolism. Vitamins like thiamine, inositol, pyridoxine, nicotinic acid act as enzymatic cofactors in the universal pathways including glycolysis and TCA cycle along with primary and secondary metabolism in the plants. The product is plant tissue culture tested but it is the sole responsibility of the user to ensure the suitability of the medium for individual species.
Application: For in Vitro Plant Cell, Tissue and organ Culture
- Appearance : White to off-white, homogeneous, free flowing powder
- Solubility: 23.2 gms/litre soluble in distilled water
- Color and Clarity: Colorless to light yellow, clear solution