M9 Minimal Salts, 5x is used in preparing M9 Minimal Medium which is used for cultivating recombinant strains Escherichia coli.
M9 Minimal Salts, 5x is a 5x concentrate that is diluted to a 1x concentration and supplemented with an appropriate carbon and energy source, such as dextrose, to provide a minimal, chemically defined medium. The medium will support the growth of “wild-type” strains of E. coli.
- Useful for maintaining positive selection pressure on plasmids coding for the ability to produce essential substances such as amino acids or vitamins
- Also used to maintain stocks of F ´-containing bacteria for use with M13
- The medium can be supplemented with specific amino acids or other metabolites, allowing for selection of specific auxotrophs
- 500 g