basic Fibroblast Growth Factors (bFGF), human recombinant, heparin-binding mitogenic proteins that enhance proliferation of a wide variety of cell types under serum-free or serum-reduced conditions. (10 µg)
Formulation:
bFGF, bovine natural - Frozen in 3.6 mM Tris, PBS (calcium, magnesium free) pH7
bFGF, human recombinant - Lyophilized from phosphate buffered saline, carrier-free; reconstitute in dH2O or serum-free medium
Preparation and Storage:
bFGF, bovine natural - Stable for at least three months at -70°C or one month solubilized at -20°C. Do not store in frost-free freezer. Avoid multiple freeze-thaws.
bFGF, human recombinant - Stable for at least three months at -20°C (lyophilized) or = one month solubilized at -20°C. Do not store in frost-free freezer. Avoid multiple freeze-thaws.
Quality:
bFGF, bovine natural
- Purity > 97% by SDS-PAGE
- Tested for ability to promote proliferation of FBHEC
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
bFGF, human recombinant
- Purity > 95% by SDS-PAGE
- Tested for ability to promote proliferation of FBHEC
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
Source:
bFGF, bovine natural - Bovine pituitary glands
bFGF, human recombinant - Recombinant, E. coli
Molecular Weight:fvx.
bFGF, bovine natural - 16 kD
bFGF, human recombinant - 17 kD
Tip:
bFGF, bovine natural - Recommended concentration - .2-20 ng/ml of medium depending on cell type
bFGF, human recombinant - Recommended concentration - 1-20 ng/ml of medium depending on cell type