A reagent for fast, simple, scalable purification of high-quality total RNA, or the simultaneous isolation of RNA, genomic DNA and protein from a wide variety of biological samples. Product Highlights Fast – simple protocol for isolation of high quality total RNA, genomic DNA…
Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted…
When yeast "a" and "alpha" cells encounter mating pheromones of the opposite cell type they induce genes necessary for mating, arrest the cell cycle in G1, alter cell surface and nuclear determinants, and also undergo dramatic morphological elongation into pear shapes,…
a-factor is one of the two mating pheromones in baking yeast. It is the “opposite” sex of mating pheromone a-Factor (alpha-factor). When yeast a and a cells encounter the opposite mating pheromones, they induce genes necessary for mating, arrest the cell cycle in G1, altering cell surface and…
TAE is used to prepare agarose gels and as an electrophoresis running buffer for the separation of double-stranded DNA in agarose and polyacrylamide gels. 50x TAE Buffer is composed of 2 M Tris-Acetate, 0.05 M EDTA, pH 8.3. For agarose gel electrophoresis, 50x TAE Buffer should be diluted to a…
Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted…
