BioAssay Systems

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BioAssay Systems

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  • For quantitative determination of chloride ion and evaluation of drug effects on chloride metabolism. Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) chloride ion. The procedure involves addition of a single working reagent and incubation for 5 min. Can readily automated…

  • Bioluminescent reagent system for rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells. High sensitivity and wide detection range. Can be readily automated on HTS liquid handling systems. The three step assay allows detection of dual…

  • Direct determination of formaldehyde concentrations in biological, food and environmental samples Safe. Non-radioactive assay. Sensitive and accurate. As low as 1.5 μM (45 ppb) formaldehyde can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No…

  • BioAssay Systems

    For quantitative determination of phosphatase activity The detection limit is generally 3 ng phosphatase or below. No wash or reagent transfer steps are involved. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405 nm. Samples: Protein phosphatases.

  • For quantitative determination of triglyceride and evaluation of drug effects on triglyceride metabolism. Linear detection range 0.01 - 1.0 mmol/L (0.88 - 88.5 mg/dL) triglyceride. Convenient room temperature high-throughput assay. Method: OD570 nm. Samples: Serum, plasma etc.

  • BioAssay Systems

    For quantitative determination of DNA. Linear detection range 2 - 40 ng (100 - 2,000 ng/mL) calf thymus DNA in 96-well plate assay. Convenient. Single working reagent. Can readily automated to test tens of thousands of samples per day. Low interference. RNA, salt (up to 3M NaCl),…

  • For quantitative determination of succinate (succinic acid) in food, beverage, agricultural products, and other biological samples. Linear detection range 10 - 400 µM for colorimetric assays and 2 - 40 µM for fluorimetric assays. Convenient room temperature assay. No 37°C…

  • For quantitative bioluminescent assay for ADP:ATP ratio (apoptosis) in cells. Safe. Non-radioactive assay. Homogeneous and convenient "mix-incubate-measure" type assay. Can be readily automated to test thousands of samples per day. Method: Luminescence. Samples: Cells.

  • For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism. Linear detection range colorimetric assay 6 to 1000 μM, fluorimetric assay 1 to 100 μM ascorbic acid. Method: OD570 nm, or FL530/585 nm. Samples: Serum, plasma,…

  • For quantitative determination of albumin. Detection range 0.3 g/dL (45 μM) to 5 g/dL (750 μM) albumin in 96-well plate assay. The procedure involves addition of a single working reagent and incubation for 5 min. Can readily automated for processing thousands of samples per day. …

  • Homogeneous assay for cell viability, proliferation, cytotoxcity and evaluation of anticancer agents Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay) Sensitive and accurate. As low as 950 cells can be accurately quantified Fast. High-throughput assay using 96-well…

  • For quantitative determination of pyruvate and evaluation of drug effects on its metabolism. Linear detection range 2 - 500 μM pyruvate for colorimetric assays and 0.2 - 50 μM for fluorimetric assays. Convenient room temperature, high-throughput assay. Method: OD570 nm, or…

  • For quantitative determination of reduced and oxidized glutathione (GSH/GSSG). Linear detection range 0.01-3 µM GSH equivalents. Method: OD412nm. Samples: Whole blood, plasma, serum, urine, tissue and cell extracts.

  • For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism. Linear detection range colorimetric assay 5 - 1000 μM, fluorimetric assay 3 - 100 μM CoA. Convenient room temperature "mix-and-read" assay. Can be readily automated to…

  • For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors. Sensitive and accurate. Detection range 10 to 600 U/L AChE activity in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the…

  • For quantitative determination of urea and evaluation of drug effects on urea metabolism. Linear detection range 0.08 -100 mg/dL (13 -17000 μM) urea in 96-well plate assay. Can be readily automated to process tens of thousands of samples per day. Method: OD520 nm. Samples: Serum,…

  • For quantitative determination of copper(II) ion and evaluation of drug effects on Cu metabolism. Linear detection range 7 μg/dL (10 μM) to 300 μg/dL (47 μM) copper. Can be readily automated to process thousands of samples per day. Assays can be executed in microplate or…

  • For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism. Linear detection range 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays. Homogeneous high-throughput assay. Method: OD570 nm,…

  • For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism. Linear detection range 50 μM to 2.5 mM glutamate. Convenient room temperature assay. No 37°C heater is needed. Can be readily automated to test…

  • For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Sensitive and accurate. Use as little as 20 μL samples. Linear detection range in 96-well plate: 0.03 to 2 mM (0.54 mg/dL to 36 mg/dL) glucose. Simple and high-throughput. The procedure…

  • For sensitive and high-throughput phosphate determination. Very stable reagent. High sensitivity and wide detection range (0.02 - 40 μM phosphate). Homogeneous "mix-and-measure" type high-throughput assay. Assays can be performed in tubes, cuvettes or microplates. …

  • For quantitative determination of glucose-6-phosphate in biological samples. Linear detection range 10 to 1000 μM G6P. Homogeneous "mix-incubate-measure" type high-throughput assay. Method: OD460 nm. Samples: Plasma, serum, tissue and culture media etc.

  • For quantitative determination of zinc ion Zn2+ and evaluation of drug effects on zinc metabolism. Linear detection range 0.12 -10 μM (0.78 - 65 μg/dL) zinc in 96-well assay format. Can be readily automated to process thousands of samples per day. Method: OD425 nm. Samples:…

  • For quantitative determination of heme and evaluation of drug effects on heme metabolism. Safe. Reagents are non-toxic. Linear detection range 0.6 - 125 μM heme. The "mix-and-read" 5 minute procedure. Can be readily automated to process tens of thousands of samples per…

  • For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism. Safe. Non-radioactive assay. Linear detection range of 1 - 150 μM bile acids. Homogeneous "mix-incubate-measure" type high-throughput assay. Method: FL530/585 nm. …

  • For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism. Linear detection range 5 - 300 mg/dL cholesterol in 96-well plate assay. Convenient. Room temperature assay. No 37°C heater is needed. Can be readily automated to process…

  • For quantitative determination of ATPase or GTPase activity and high-throughput screen for their inhibitors. High sensitivity: detection of 0.007 U/L ATPase or GTPase activity. Fast and convenient: single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme…

  • For quantitative determination of L-phenylalanine and evaluation of drug effects on its metabolism. Safe. Non-radioactive assay. Linear detection range of 2 - 300 μM L-phenylalanine. Can be readily automated to test thousands of samples per day. Method: FL530/595 nm. Samples:…

  • Indole determination in biological samples. Linear detection range 3 - 100 µM indole in 96-well plate assay. The procedure involves adding a single working reagent, and reading the absorbance immediately. Method: OD565 nm. Samples: Bacterial growth medium.

  • For quantitative determination of α-mannosidase enzyme activity using a p-nitrophenol based substrate. Fast and sensitive. Linear detection range (10 μL sample): 1 to 250 U/L for a 10 minute reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure"…

  • For quantitative determination of ethanol and evaluation of drug effects on alcohol metabolism. Linear detection range 0.0008 - 0.1 vol % (140 - 17,500 μM, or 8 -1000 ppm) in 96-well plate assay. Can be readily automated to process thousands of samples per day. Method: OD565 nm…

  • MPO peroxidation activity determination in biological samples (e.g. cell lysates, tissues, etc.) Linear detection range 0.0025 to 2 U/L. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to test thousands of samples per day. Method: FL530/585 nm. …

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