The TGFβ/SMAD reporter lentivirus is designed for monitoring the activity of the TGFβ/SMAD signaling pathway. The transforming growth factor beta (TGFβ) signaling pathway is involved in many cellular processes in both the adult organism and the developing embryo including cell…

It has been observed that microRNA miR-182 plays a role in tumorigenesis and cancer metastasis. This lentivirus expresses miR-182 under the control of the Tet promoter.

Luciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. Firefly luciferase refers to the enzyme that was isolated from Photinus pyralis. This lentivirus expresses Firefly luciferase under the control of CMV promoter.

Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…

RAR-related orphan receptor B (RORB) is a member of the NR1 subfamily of nuclear hormone receptors. Rorb has been shown to be involved in the differentiation of rod photoreceptor cells, Amacrine and horizontal interneurons and associated with bipolar disorder. This lentivirus expresses human RORB…

Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…

Luciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. Firefly luciferase refers to the enzyme that was isolated from Photinus pyralis.

Cre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…

NANOG is a transcription factor important for maintaining the self-renewal capacity of undifferentiated stem cells. Ectopic expression of NANOG has been used to generate iPS cells in culture. Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used…

SOX9 is a transcription factor essential for multipotent neural stem cell (NSC) formation and maintenance. Sox9 is a major molecular component of the neuron–glia switch in the developing spinal cord. SOX9 also plays a key role in chondrogenesis. This lentivirus expresses human SOX9 under the…

Telomerase reverse transcriptase (TERT, or hTERT in human) is a catalytic subunit of the enzyme telomerase, Ectopic expression of telomerase in telomerase-silent cells is sufficient to overcome senescence and to extend cellular lifespan, and has been used for cell immortalization. This lentivirus…

The basic motif-leucine zipper transcription factor NRL (neural retina leucine zipper) is essential for rod photoreceptor specification in the mammalian retina. This lentivirus expresses human Nrl under the control of the Tet promoter.

This strain carries the tetracycline reverse transcriptional activator (rtTA) under the control of the CMV promoter. The rtTA binds to tetracycline operator elements (TetO) in the presence of doxycycline, causing transcriptional activation of genes downstream of TetO.

Angpt1 gene encodes a secreted glycoprotein called Angiopoietin 1 that activates the receptor by inducing its tyrosine phosphorylation. It appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It may also mediate blood…

OTX2 is a homeodomain transcription factor and one of the key regulator genes of photoreceptor formation. This lentivirus expresses human OTX2 under the control of the Tet promoter.

Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…

This is a vector control for our EF1A lentiviral vectors.

This is a vector control for our CMV lentiviral vectors.

Hnf4a is a nuclear transcription factor which binds DNA as a homodimer. Hnf4a controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver,…

ASCL1 (Achaete-Scute Family BHLH Transcription Factor 1) is a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding.…

TurboRFP is a red (orange) fluorescent protein (ex. 553 and em. 574 nm) derived from sea anemoneEntacmaea quadricolor [Merzlyak et al., 2007]. Possessing high photostability and pH stability, TurboRFP is more than twice brighter than DsRed2. Fast TurboRFP maturation makes it clearly detectable in…

PR domain containing 1 with zinc finger domain (PRDM1)/B lymphocyte–induced maturation protein 1 (BLIMP1) is a transcriptional repressor. PRDM1 plays fundamentally important roles in many cell lineages and in the early development of several cell types, including B and T lymphocytes, germ…

MAD2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2 was showed to be associated with tumor differentation. This lentivirus express human MAD2 under the control of the CMV promoter.

ANG gene encodes a mediator of new blood vessel formation that hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. It interacts with DNA causing a promoter-like increase in the expression of rRNA. Angiogenic activity is regulated by…

Ars2 (arsenite-resistance protein 2; also known as Srrt) is a highly conserved zinc finger protein. Ars2 is necessary and sufficient to promote NSC (neural stem cells) self-renewal. This premade lentivirus expresses Ars2 under the control of Tet promoter.

OTX2 is a homeodomain transcription factor and one of the key regulator genes of photoreceptor formation. This lentivirus expresses human OTX2 under the control of the CMV promoter.

The homeodomain transcription factor Chx10 is one of the earliest markers of the developing retina. It is required for retinal progenitor cell proliferation as well as formation of bipolar cells, a type of retinal interneuron.

It has been observed that microRNA miR-183 plays an important role in tumorigenesis and cancer metastasis. This lentivirus expresses miR-183 under the control of the Tet promoter.

OCT3/4 (POU5F1) is a transcription factor that is expressed in stem cells and germ cells. Its expression is required to sustain cell self-renewal of undifferentiated stem cells. Oct-3/4 is the most recognized marker for totipotent ES cells. Ectopic expression of OCT3/4 has been used to produce iPS…

RAX (Retina And Anterior Neural Fold Homeobox) is a homeobox-containing transcription factor that functions in eye development. RAX is required for retinal cell fate determination and also regulates stem cell proliferation. Mutations in this gene have been reported in patients with defects in…

Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…

This set contains four CMV-driven vectors: SOX2 (PLV-10008), OCT3/4 (PLV-10007), KLF4 (PLV-10006) and C-MYC (PLV-10005).