12352200, bioWORLD, 250 nmol
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DeoxyInosine
bioWORLDHistorically, the first universal base employed was 2’-deoxyInosine (dI). DeoxyInosine is a naturally occurring base that, while not truly universal, is less destabilizing than mismatches involving the four standard bases. Hydrogen bond interactions between dI and dA, dG, dC and…
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DeoxyUridine
bioWORLDDeoxyUridine (dU) can be substituted for dT in DNA oligonucleotides. The base can be removed by the enzyme uracil-Ndeglycosylase (UNG), which renders the oligo susceptible to strand scission. One common use of this strategy is to eliminate amplifi ed DNA and prevent cross-contamination. ChEBI:…
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