Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic, and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.
Potassium phosphate typically used as a component for a wide variety of media used in the culture of microorganisms, as a component in phosphate buffered saline (PBS). In addition to helping maintain pH, it supplies essential phosphate. The use of high concentrations of potassium phosphate in the immobilization of affinity ligands onto epoxide-activated stationary phases has been reviewed. A two-phase system of aqueous potassium phosphate and poly(ethylene glycol) for the isolation of E. coli β-galactosidase and β-galactosidase fusion proteins has been published. The quantitation of nonionic surfactants in buffered solutions using strong cation and anion exchange HPLC guard columns and potassium phosphate solution has been investigated.
Purity: ≥99%
Key Applications: Biological Buffers, Buffers, Chemical Synthesis
Application Areas: Molecular Biology, Cell Biology
Product Type: Inorganics
Biochemical Category: Biochemicals
Presentation: Clear Granular Crystals
Format: Granular Crystals
NOTES: For phosphate buffers, the pH increases with decreasing temperature. Compared with a buffer at 25°C, buffers at 4°C will be 0.08 pH unit higher and a buffer at 37°C will be 0.025 pH unit lower. The concentration of phosphate also influences pH.
Typical Buffer Preparation/Formulations: 1 M Potassium Phosphate Monobasic, 1 M Potassium Phosphate Dibasic, 5 M Sodium Chloride
Solubility: Soluble in water (100 mg/mL) ; Insoluble to slightly soluble in ethanol; insoluble in alcohol.
Storage & Handling: Store at Room Temperature (15-30°C)